Normalization - RPKM/FPKM/TPM

What is you favorite dividend?

Normalization is essential when comes to statistics. Absolute quantification is not wrong but more of the time we are more capable to understand the graph when control is always 1. In the case of RNA-Seq, to make it super simple, the main stream at the time of writing is TPM and if you are using DESeq2 or edgeR for the differential expression analysis you are automatically using TPM.　 I strongly recommend to watch this blog-post for a deeper understanding of the normalization model.

https://www.rna-seqblog.com/rpkm-fpkm-and-tpm-clearly-explained