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NGS for natural scientist
  • 1. Preface
    • How to use this book
    • Motivation
    • Genomic data science as a tool to biologist
    • Next Generation Science (also NGS)
  • 2. Getting started
    • A step by step pipeline tutorial
    • Sequencing chemistry explained by Illumina
    • Joining a course
    • RNA quality and Library prep
    • (optional) My click moment about "Why Linux"
  • 3. Good-to-know beforehand
    • Experiment design
    • Single-end and Paired-end
    • Read per sample and data size
    • Normalization - RPKM/FPKM/TPM
    • Gene annotation
  • 4. Setting up terminal
    • My Linux terminal
    • Linux environment
    • R and RStudio
    • PATH
  • 5. FASTQ and quality control
    • Getting FASTQ files from online database
    • FASTQ quality assessment
  • 6. Mapping/alignment and quantification
    • Salmon
    • DESeq2
  • 7. Visualization
  • 8. Single cell RNA-Seq
  • 9. AWS cloud and Machine Learning
    • Machine Learning in a nutshell
    • R vs Python
    • Setting up ML terminal
    • Data exploration
  • (pending material)
    • graphPad
    • readings for ML
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  1. 1. Preface

Next Generation Science (also NGS)

Taking things to another level.

PreviousGenomic data science as a tool to biologistNext2. Getting started

Last updated 1 year ago

From the moment I started this book, to the point I started on this page, there was exactly 2 years in between, and I reckon Summer is my favourite time in the year because most of my beautiful moments are hot and sunny. For some reason life guides me to a more sophisticated computational work to practice science, and because of everything - more affordable cloud infrastructure, resources accessibility over internet, my job nature, no-code environment, digitial era of biological research - to practice science consultance at molecular level and for basic R&D has become so much feasible than ever, which justified my learning beyond sequencing to ML/AI, and in the future, quantum computing.

This is where I started on section , where I invented a new meaning for NGS - Next Generation Science (in my book).

9. AWS cloud and ML